Background: Gastroenteritis caused by bacteria are a serious public health issue. Antibiotic resistance is common. This work described the epidemiological and resistance profile of bacteria involved in gastrointestinal infection in Douala, Cameroon. Methods: A cross-sectional study was conducted from March to December 2017 on stool samples. Bacterial species were diagnosed on the basis of morphological, biochemical and physiological tests. The resistance profile on fifteen routine antibiotics was investigated using the disks diffusion method. Results: Out of 250 stool samples examined, 206 were positive, 129 (62.62%) samples had only one bacterial species and 77 (37.38%) more than one species. 301 pathogenic bacteria were identified of which Escherichia coli represented more than one third (33.89%), followed by Klebsiella pneumoniae (22.26%), Enterobacter aerogenes (11.96%), Salmonella sp (10.30%), Citrobacter freundii (9.97%), Yersinia enterocolitica (4.65%), Shigella flexneri (3.99%), Serratia marcescens (2.33%) and Proteus sp (0.66%). The antibiogram showed high resistance to Tetracycline, Amoxycillin, Amoxycillin + clavulanic acid, Doxycycline and Cotrimoxazol. Imipenem and Amikacin were the most active Conclusion: The present findings provide additional information for the control of gastrointestinal tract infections in Douala.
| Published in | International Journal of Gastroenterology (Volume 5, Issue 1) |
| DOI | 10.11648/j.ijg.20210501.12 |
| Page(s) | 5-17 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
Epidemiology, Gram- Bacteria, Gastroenteritis, Antibiotic Resistance, Douala, Cameroon
| [1] | Bahramian A, Khoshnood S, Shariati A, Doustdr F, Chirani A. S, Heidary M (2019). Molecular characterization of the pilS2 gene and its association with the frequency of Pseudomonas aeruginosa plasmid pKKLC102 and PAP1-1 pathogenicity Island. Infection and Drug Resistance. 12: 221-227. |
| [2] | Maleki D. T, Ghalavand Z, LaabeI Nikmaneh H. H, Kodori M, Haxhemi et al (2019). Molecular analysis of accessory gene regulator functionality and virulence genes in S. aureus derived from pediatric wound infections. Infection Genetics and Evolution. 73: 255-260. |
| [3] | WHO: Global antimicrobial resistance surveillance system (GLASS) report: early implementation. 2017-2018 http://wwww hoint/drug resistance/surveillance/GLASS meeting/en/. |
| [4] | Azimi T, Saied M, Fallah F, Azimi L Gholinejad Z (2019). Evaluating the antimicrobial resistance patterns among major bacterial pathogens isolated from clinical specimens taken from patients in Mofid Children’s Hospital, Tehran, Iran 2013–2018. Infection and Drug Resistance. 12: 2089–2102. |
| [5] | Dougnon V, Assogba P, Gnimatin J. P, Agbankpé J, Koudokpon H, Deguenon E (2020). Modeling the Antimicrobial Resistance of Enterobacteria Responsible for Urinary Tract Infections in Benin: Another Way to Control Antimicrobial Resistance. Asian Journal of Biochemistry, Genetics and Molecular Biology. 3: 48-61. |
| [6] | Azimi T, Nasiri M. J, Zamani S, Hashemi A, Goudarzi H, Imani F. A. A, et al (2018). High genetic diversity among Mycobacterium tuberculosis strains in Tehran. Iranian Journal of Clinical Tuberculosis and other Mycobacterial Diseases. 11: 1-6. |
| [7] | Aslam B, Wang W, Arshad I. M, Khursshid M, Muzammil S et al (2018). Antibiotic resistance: a rundown of a global crisis. Infection and Drug Resistance. 11: 1645-1658. |
| [8] | Nasser A, Jazireian P, Safari H, Alizade-Sani M, Pourmand MR, Azimi T (2019). Staphylococcus aureus versus neutrophil: scrutiny of ancient combat. Microbial Pathogenesis. 131: 259–269. |
| [9] | Tsutsui A, Suzuki S (2018). Japan nosocomial infections surveillance (JANIS) A model of sustainable national antimicrobial resistance surveillance based on hospital diagnostic microbiology laboratories. BMC Health Services Research. 18 (1): 799. |
| [10] | Li L, Dai J. X, Chen Z. H, Li Y, Liu M, Wen Y, et al (2018). Antimicrobial resistance and pathogen distribution in hospitalized burn patients: a multicenter study in southeast China. Medecine. 97: 34. |
| [11] | Gangoue-Piéboji J, Koulla-Shiro S, Ngassam P,. Adiogo D, Ndumbe P (2006). Antimicrobial activity against gram negative bacilli from Yaounde Central Hospital, Cameroon. African Heath Sciences. 6 (4): 232-237. |
| [12] | Ngueguim F (2013). Prévalence des Bactéries Gram-Négatif à Phénotypes Multi-Résistants chez les Patients souffrant d´infections du Tractus Entéro-Gastrique à l´Hôpital de District de Dschang et Rôle des Associations Des Antibiotiques. Thèse de master of science en biochimie clinique. Université de Dschang Cameroun. 104p. |
| [13] | Ebongue C. O, Tsiazok M. D, Mefo'o J. P. N, Ngaba G. P, Beyiha G, Adiogo D (2015). Evolution of antibiotic resistance in isolated Enterobacteriaceae at the Douala General Hospital from 2005 to 2012. Panafrican Medical Jounal. 20: 227. |
| [14] | REMIC/SFM 2015, Référentiel en microbiologie médicale Tomes 1 et 2, 5eme édition. |
| [15] | Marbou W. J. T, Kuete V (2017). Bacterial resistance and immunological profiles in HIV− infected and non-infected patients at Mbouda AD LUCEM Hospital in Cameroon. Journal of. Infections and Public Health. 10: 269–276. |
| [16] | Sociéte Francaise de Microbiologie 2015. (page téléchargée le 15/02/2016). Comité de l'antibiogramme: Recommandations. http://http://www.sfm.asso.fr/. Google Scholar. |
| [17] | Ngalani J. T. O, Mbaveng A. T, Marbou W. J. T, Ngai R. Y, Kuete V (2019). Antibiotic Resistance of Enteric Bacteria in HIV-Infected Patients at the Banka Ad-Lucem Hospital, West Region of Cameroon. Canadian. Journal of. Infectious. Disesases and Medical Microbiology. 7p. |
| [18] | Mahmoudi S, Mahzari M, Banar M, Pourakbari B, Ashtriani H. M. T, Mohammadi M, et al (2017). Antimicrobial resistance patterns of Gram-negative bacteria isolated from blood stream infections in an Iranian referral paediatric hospital: a 5.5-year study. Journal of Global Antimicrobial Resistance. 11: 17–22. |
| [19] | Moutachakkir M, Chinbo M, Elkhoudri N, Soraa N (2015). Antibiotic resistance in uropathogenic enterobacteriaceae in pediatric settings at the CHU in Marrakech. Journal Pediatrics and Child care. 28: 16–22. |
| [20] | Ako A. A, Nkeng G. E, Takem G. E. E (2009). Water quality and occurence of water- borne diseases in the Douala 4th District, Cameroon. Water Sciences and Technology. 59 (12): 2321-2330. |
| [21] | Dyar O. J, Hoa N. Q, Trung N. V, Phuc H. D, Larsson M, Chuc T. K. N et al (2012). High Prevalence of antibiotic resistance in commensal Escherichia coli among children in rural Vietnam. BMC infectious Deseases. 12 (1): 92. |
| [22] | Kweyang B. P. T, Kwetche P. R. F, Njine T, Nola M, Djogo P (2009). Quelques facteurs déterminant la distribution des bactéries du genre Vibrio dans l'environnement aquatique de Yaoundé, Cameroun. Cameroon Journal of Experimental. Biology. 5 (2): 96-103. |
| [23] | Meremo A, Mshana S. E, Kidenya B. R, Kabangila R, Peck R, Kataraihya J. B (2012). High prevalence of non-typhoid salmonella bacteraemia among febrile HIV adult patients admitted at a tertiary hospital, North-Western Tanzania. International Archives of Medicine. 5 (1): 28. |
| [24] | Konare M. S. Antibiotic sensitivity of enterobacteriaceae strains isolated in 2016 at the Laboratory of Medical Biology and Hospital Hygiene at CHU du Point G, 2016. |
| [25] | Kuete V, Ngameni B, Tangmouo G, Bolla J, Alibert-Franco S, Ngadjui T, Pagès J (2010). Efflux Pumps Are Involved in the Defense of Gram-Negative Bacteria against the Natural Products Isobavachalcone and Diospyrone. Antimicrobial. Agents Chemotherapy. 54: 1749–1752. |
| [26] | Inan A, Ozgultekin A, Akcay SS, Engin D. O, Turan G, Ceran N, Dincer E, Aksaray S, Goktas P, Erdem I (2012). Alterations in bacterial spectrum and increasing resistance rates in isolated microorganisms from device-associated infections in an intensive care unit of a Teaching Hospital in Istanbul (2004-2010). Japanese Journal of Infectious Diseases. 65 (2): 146–151. |
| [27] | Hæggman S, Lo¨fdahl S, Paauw L. A, Verhoef J, Briss S (2004). Diversity and Evolution of the Class A Chromosomal Beta-Lactamase Gene in Klebsiella pneumoniae. Antimicrobial. Agents Chemotherapy. 48 (7): 2400–2408. |
| [28] | Davin-Regli A, Chollet R, Boll C, Pagès J (2004). Les multi-résistances et leurs mécanismes chez Enterobacter aerogenes. Antibiotherapy. 6 (3): 202-206. |
| [29] | Gassama A, Thiaw B, Dia N. M, Fall F, Camara P, Hovette P, Perret J. L, et al (2000). Etiologies infectieuses des diarrhées au cours du SIDA de l’adulte à Dakar: Etude cas/Témoins sur 594 malades. Dakar Medical. 46: 46-50. |
| [30] | CLSI: Performance Standards for Antimicrobial Susceptibility Testing, Clinical and Laboratory Standards Institute, Wayne, PA, USA 27th edition, 2017, https://clsi.org/media/1469/m100s27_sample.pdf. |
| [31] | Boutiba I, Boubaker B, Ghozzi R, Jouaihia W, Mahjoubi F,. Thabet L et al (2007). Résistance bactérienne aux antibiotiques en Tunisie: données de 1999 à 2003. Revue Tunisienne d'Infectiologie. 1 (4): 5-11. |
| [32] | Hashemi S. H, Esna-Ashari F, Tavakoli S, Mamani M (2013). The prevalence of antibiotic Resistance of Enterobacteriaceae strains isolated in community and Hospital acquired in infections in teaching hospital of Hamadan, west of Iranian. Journal of Research in Health Sciences. 13 (1): 75-80. |
APA Style
Raphael Tchientcheu, Calixte Ida Penda, Rebecca Madeleine Ebelle Etame, Colette Grace Ngondi Dalle, Raymond Simplice Mouokeu, et al. (2021). Epidemiology and Sensibility Profile of Some Enteric Bacteria Isolated from Stool Samples in Some Douala-Cameroon Hospitals. International Journal of Gastroenterology, 5(1), 5-17. https://doi.org/10.11648/j.ijg.20210501.12
ACS Style
Raphael Tchientcheu; Calixte Ida Penda; Rebecca Madeleine Ebelle Etame; Colette Grace Ngondi Dalle; Raymond Simplice Mouokeu, et al. Epidemiology and Sensibility Profile of Some Enteric Bacteria Isolated from Stool Samples in Some Douala-Cameroon Hospitals. Int. J. Gastroenterol. 2021, 5(1), 5-17. doi: 10.11648/j.ijg.20210501.12
AMA Style
Raphael Tchientcheu, Calixte Ida Penda, Rebecca Madeleine Ebelle Etame, Colette Grace Ngondi Dalle, Raymond Simplice Mouokeu, et al. Epidemiology and Sensibility Profile of Some Enteric Bacteria Isolated from Stool Samples in Some Douala-Cameroon Hospitals. Int J Gastroenterol. 2021;5(1):5-17. doi: 10.11648/j.ijg.20210501.12
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.ijg.20210501.12,
author = {Raphael Tchientcheu and Calixte Ida Penda and Rebecca Madeleine Ebelle Etame and Colette Grace Ngondi Dalle and Raymond Simplice Mouokeu and Rosalie Anne Ngono Ngane},
title = {Epidemiology and Sensibility Profile of Some Enteric Bacteria Isolated from Stool Samples in Some Douala-Cameroon Hospitals},
journal = {International Journal of Gastroenterology},
volume = {5},
number = {1},
pages = {5-17},
doi = {10.11648/j.ijg.20210501.12},
url = {https://doi.org/10.11648/j.ijg.20210501.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijg.20210501.12},
abstract = {Background: Gastroenteritis caused by bacteria are a serious public health issue. Antibiotic resistance is common. This work described the epidemiological and resistance profile of bacteria involved in gastrointestinal infection in Douala, Cameroon. Methods: A cross-sectional study was conducted from March to December 2017 on stool samples. Bacterial species were diagnosed on the basis of morphological, biochemical and physiological tests. The resistance profile on fifteen routine antibiotics was investigated using the disks diffusion method. Results: Out of 250 stool samples examined, 206 were positive, 129 (62.62%) samples had only one bacterial species and 77 (37.38%) more than one species. 301 pathogenic bacteria were identified of which Escherichia coli represented more than one third (33.89%), followed by Klebsiella pneumoniae (22.26%), Enterobacter aerogenes (11.96%), Salmonella sp (10.30%), Citrobacter freundii (9.97%), Yersinia enterocolitica (4.65%), Shigella flexneri (3.99%), Serratia marcescens (2.33%) and Proteus sp (0.66%). The antibiogram showed high resistance to Tetracycline, Amoxycillin, Amoxycillin + clavulanic acid, Doxycycline and Cotrimoxazol. Imipenem and Amikacin were the most active Conclusion: The present findings provide additional information for the control of gastrointestinal tract infections in Douala.},
year = {2021}
}
TY - JOUR T1 - Epidemiology and Sensibility Profile of Some Enteric Bacteria Isolated from Stool Samples in Some Douala-Cameroon Hospitals AU - Raphael Tchientcheu AU - Calixte Ida Penda AU - Rebecca Madeleine Ebelle Etame AU - Colette Grace Ngondi Dalle AU - Raymond Simplice Mouokeu AU - Rosalie Anne Ngono Ngane Y1 - 2021/01/22 PY - 2021 N1 - https://doi.org/10.11648/j.ijg.20210501.12 DO - 10.11648/j.ijg.20210501.12 T2 - International Journal of Gastroenterology JF - International Journal of Gastroenterology JO - International Journal of Gastroenterology SP - 5 EP - 17 PB - Science Publishing Group SN - 2640-169X UR - https://doi.org/10.11648/j.ijg.20210501.12 AB - Background: Gastroenteritis caused by bacteria are a serious public health issue. Antibiotic resistance is common. This work described the epidemiological and resistance profile of bacteria involved in gastrointestinal infection in Douala, Cameroon. Methods: A cross-sectional study was conducted from March to December 2017 on stool samples. Bacterial species were diagnosed on the basis of morphological, biochemical and physiological tests. The resistance profile on fifteen routine antibiotics was investigated using the disks diffusion method. Results: Out of 250 stool samples examined, 206 were positive, 129 (62.62%) samples had only one bacterial species and 77 (37.38%) more than one species. 301 pathogenic bacteria were identified of which Escherichia coli represented more than one third (33.89%), followed by Klebsiella pneumoniae (22.26%), Enterobacter aerogenes (11.96%), Salmonella sp (10.30%), Citrobacter freundii (9.97%), Yersinia enterocolitica (4.65%), Shigella flexneri (3.99%), Serratia marcescens (2.33%) and Proteus sp (0.66%). The antibiogram showed high resistance to Tetracycline, Amoxycillin, Amoxycillin + clavulanic acid, Doxycycline and Cotrimoxazol. Imipenem and Amikacin were the most active Conclusion: The present findings provide additional information for the control of gastrointestinal tract infections in Douala. VL - 5 IS - 1 ER -
Email: